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Endpoint: Skin Corrosivity
Endpoints: Skin and Eye Irritation
Skin Irritancy Tests using Human Skin Models
Ocular Irritancy Ex Vivo
Endpoint: Mucosal Irritancy
Endpoint: Phototoxicity Testing
Endpoint: In Vitro Dermal Penetration
Endpoint: Skin (Contact) Sensitisation
QSAR
Future Prospects

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Alternative Toxicology - Ocular Irritancy Ex Vivo

The Rabbit Enucleated Eye Test (REET)

This test assesses the ocular irritation potential of a test substance following topical application onto the surface of the cornea of enucleated rabbit eyes.

It has been used routinely at SPL since 1999 and has resulted in large savings in the number of live animals required for determination of the eye irritancy of chemicals. The eyes (pre and post treatment) are maintained in a superfusion chamber at 32ºC. This ensures that the eyes remain viable throughout the duration of the test.

The direct effect of the test substance on the cornea can be assessed using multi-endpoint analysis following evaluation of changes in corneal thickness, corneal opacity, alteration of the corneal epithelium and fluorescein uptake up to 240 minutes following treatment.

 


The REET has been shown to reliably differentiate between negligible and moderate to severe irritants.
 
The US National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) in collaboration with the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) is currently reviewing the validation status of the REET, together with three other methods [the bovine corneal opacity (BCOP) test, the isolated chicken eye (ICE) or chicken enucleated eye test (CEET), and the hen's egg test chorionallantoic membrane (HET CAM)] for assessing ocular irritation / corrosion. The REET is currently being has not yet undergone formal validation although it should be noted that a positive result in the test will be accepted by most regulatory authorities without the need for additional confirmatory testing.

 

 

 

 

 

 

 

 

 

 
 
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